Value of vascular cell adhesion molecule-1 and CC16 protein in bronchiolitis in young children
Keywords:bronchiolitis, children, early age, allergic history, vascular cell adhesion molecule-1, CC16 protein
Given the severity of the course of bronchiolitis in young children, especially with a burdened allergic history, the search for biomarkers for early diagnosis and prognosis of the disease is urgent.
Purpose - to substantiate the expediency of determining the levels of VCAM-1 and CC16 in blood serum in bronchiolitis in young children.
Material and methods. The main group consisted of 34 children with bronchiolitis without a serious allergic history. The comparison group included 33 children who suffered from bronchiolitis and had a severe allergic history. The control group consisted of 25 conditionally healthy children. The clinical and laboratory examination consisted in determining the levels of VCAM-1 and CC16 in blood serum using the immunoenzymatic method.
Results. The level of VCAM-1 in blood serum was established (55.5±1.12 ng/ml) in the children of the main group, while in the children of the comparison group the average value (164.9±1.1 ng/ml) of this indicator was 3 times higher, (AOR=3; 95% CI: 1.48-6.12; p<0.05). In children of the control group, the average level of VCAM-1 in blood serum was (41.6±1.32 ng/ml). Analysis of the average level of CC16 protein in blood serum revealed its significant increase in children of the main group (41.2±1.24 ng/ml) compared to the indicator of children with bronchiolitis with a severe allergic history (22.8±1.64 ng/ml ml), (AOR=1.6; 95% CI: 0.8-3.2; p<0.05). In children of the control group, the average value of the CC16 protein was within the reference values (16.2±2.43 ng/ml).
Conclusions. In young children with bronchiolitis, it is advisable to determine the level of CC16 and VCAM-1 protein in blood serum. It was found that the level of SS16 protein in blood serum was significantly lower in children with a severe allergic history compared to children without a severe allergic history. On the other hand, regarding the level of VCAM-1 in blood serum, its value was significantly increased in the majority of children with severe allergic history.
The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of all participating institutions. The informed consent of the patient was obtained for conducting the studies.
No conflict of interests was declared by the authors.
Almuntashiri S, Zhu Y, Han Y, Wang X, Somanath PR, Zhang D. (2020). Club Cell Secreted Protein CC16: Potential Applications in Prognosis and Therapy for Pulmonary Diseases. Journal of clinical medicine. 9 (12): 4039. https://doi.org/10.3390/jcm9124039; PMid:33327505 PMCid:PMC7764992
Birnhuber A, Fliesser E, Gorkiewicz G, Zacharias M, Seeliger B, David S et al. (2021). Between inflammation and thrombosis: endothelial cells in COVID-19. European Respiratory Journal. 58 (3): 2100377. https://doi.org/10.1183/13993003.00377-2021; PMid:33958433 PMCid:PMC8112008
Cui A, Xiang M, Xu M, Lu P, Wang S, Zou Y et al. (2019). VCAM-1-mediated neutrophil infiltration exacerbates ambient fine particle-induced lung injury. Toxicology Letters. 302: 60-74. https://doi.org/10.1016/j.toxlet.2018.11.002; PMid:30447258
Fukumoto J, Soundararajan R, Leung J, Cox R, Mahendrasah S, Muthavarapu N et al. (2016). The role of club cell phenoconversion and migration in idiopathic pulmonary fibrosis. Aging. 8: 3091-3109. https://doi.org/10.18632/aging.101115; PMid:27899769 PMCid:PMC5191887
Genies MC, Kim JM, Pyclik K, Rossi S, Spicyn N, Serwint JR. (2018). Impact of an educational intervention to improve physician adherence to bronchiolitis clinical practice guidelines: a pre-post intervention study. Clinical pediatrics. 57 (3): 253-258. https://doi.org/10.24061/2413-4260.XII.1.43.2022.6
Guerra S, Vasquez MM, Spangenberg A, Halonen M, Martin RJ. (2016). Club cell secretory protein in serum and bronchoalveolar lavage of patients with asthma. J. Allergy Clin. Immunol. 138: 932-934.e1. https://doi.org/10.1016/j.jaci.2016.03.047; PMid:27315766 PMCid:PMC5014567
Guzmán-Bárcenas J, Calderón-Moore A, Baptista-González H, Irles C. (2017). Clara Cell Protein Expression in Mechanically Ventilated Term and Preterm Infants with Respiratory Distress Syndrome and at Risk of Bronchopulmonary Dysplasia: A Pilot Study. Can. Respir. J: 1-5. https://doi.org/10.1155/2017/8074678; PMid:28487624 PMCid:PMC5405359
Hong EY, Ahn YM, Hong G, Ro JY. (2019). A role of mast cells in ovalbumin-induced mouse allergic asthma responses suppressed by natural product mixture (Hwangchango). Journal of Allergy and Clinical Immunology. 143 (2): AB74. https://doi.org/10.1016/j.jaci.2018.12.230
Hunderi J, Rolfsjord LB, Carlsen K, Holst R, Bakkeheim E, Berents TL et al. (2020). Virus, allergic sensitisation and cortisol in infant bronchiolitis and risk of early asthma. ERJ open research. 6 (1): 00268-2019. https://doi.org/10.1183/23120541.00268-2019; PMid:32201686 PMCid:PMC7073413
Kou M, Hwang V, Ramkellawan N. (2018). Bronchiolitis: From practice guideline to clinical practice. Emergency Medicine Clinics. 36 (2): 275-286. https://doi.org/10.1016/j.emc.2017.12.006; PMid:29622322
Li G, Jiang X, Liang X, Hou Y, Zang J, Zhu B et al. (2023). BAP31 regulates the expression of ICAM-1/VCAM-1 via MyD88/NF-κB pathway in acute lung injury mice model. Life Sciences. 313: 121310. https://doi.org/10.1016/j.lfs.2022.121310; PMid:36549351
Li Y, Huang X, Guo F, Lei T, Li S, Monaghan-Nichols P et al. (2020). TRIM65 E3 ligase targets VCAM-1 degradation to limit LPS-induced lung inflammation. Journal of molecular cell biology. 12 (3): 190-201. https://doi.org/10.1093/jmcb/mjz077; PMid:31310649 PMCid:PMC7181722
Liu M, Lu J, Zhang Q, Zhang Y, Guo Z. (2021). Clara cell 16 KDa protein mitigates house dust mite-induced airway inflammation and damage via regulating airway epithelial cell apoptosis in a manner dependent on HMGB1-mediated signaling inhibition. Molecular medicine (Cambridge, Mass.). 27 (1): 11. https://doi.org/10.1186/s10020-021-00277-4; PMid:33541260 PMCid:PMC7863538
Perkins TN, Oczypok EA, Milutinovic PS, Dutz RE, Oury TD. (2019). RAGE-dependent VCAM-1 expression in the lung endothelium mediates IL-33-induced allergic airway inflammation. Allergy. 74 (1): 89-99. https://doi.org/10.1111/all.13500; PMid:29900561
Rokicki WM, Wojtacha J, Dżeljijli A. (2016). The role and importance of club cells (Clara cells) in the pathogenesis of some respiratory diseases. Pol. J. Cardio-Thoracic Surg. 1: 26-30. https://doi.org/10.5114/kitp.2016.58961; PMid:27212975 PMCid:PMC4860431
Sonntag H-J, Filippi S, Pipis S, Custovic A. (2019). Blood Biomarkers of Sensitization and Asthma. Front. Pediatr. 7: 251. https://doi.org/10.3389/fped.2019.00251; PMid:31275911 PMCid:PMC6593482
Spella M, Lilis I, Stathopoulos GT. (2017). Shared epithelial pathways to lung repair and disease. Eur. Respir. Rev. 26: 170048. https://doi.org/10.1183/16000617.0048-2017; PMid:28659498 PMCid:PMC9488986
Tokarchuk N, Overchuk A. (2022). The significance of CC16 protein in bronchiolitis in young children. Neonatology, surgery and perinatal medicine. 12; 2 (44): 34-37. https://doi.org/10.24061/2413-4260.XII.2.44.2022.6
Tokarchuk N, Overchuk A. (2022). Diagnostic value of markers of allergic inflammation in bronchiolitis in infants. Neonatology, surgery and perinatal medicine. 12; 1 (43): 31-36. https://doi.org/10.24061/2413-4260.XII.1.43.2022.6
Copyright (c) 2023 Modern pediatrics. Ukraine
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The policy of the Journal “MODERN PEDIATRICS. UKRAINE” is compatible with the vast majority of funders' of open access and self-archiving policies. The journal provides immediate open access route being convinced that everyone – not only scientists - can benefit from research results, and publishes articles exclusively under open access distribution, with a Creative Commons Attribution-Noncommercial 4.0 international license (СС BY-NC).
Authors transfer the copyright to the Journal “MODERN PEDIATRICS. UKRAINE” when the manuscript is accepted for publication. Authors declare that this manuscript has not been published nor is under simultaneous consideration for publication elsewhere. After publication, the articles become freely available on-line to the public.
Readers have the right to use, distribute, and reproduce articles in any medium, provided the articles and the journal are properly cited.
The use of published materials for commercial purposes is strongly prohibited.