The von Willebrand factor as a marker of partially controlled asthma severity in children
Keywords:asthma, children, endothelial dysfunction, von Willebrand factor
Asthma is one of the most common chronic non-communicable diseases among adults and children. Recent studies have paid special attention to endothelial dysfunction in the mechanism of development and progression of asthma, on the one hand, and the occurrence of long-term consequences of endothelial damage, on the other hand. Endothelial dysfunction in the modern sense is not only a pathology of the vascular wall but also a deep, complexly organized system of disorders and compensatory and adaptive reactions that originates at the molecular genetic level.
Purpose - to improve the knowledge of assessing the levels of von Willebrand factor (VWF) as a marker of endothelial dysfunction in the blood of children with partially controlled asthma.
Materials and methods. 94 children participated in the study. Patients were divided into 4 groups: the Group 1 - children with mild persistent asthma (n=59), the Group 2 - moderately severe persistent asthma (n=10), the Group 3 - severe persistent asthma (n=12), and the Group 4 - control group (n=13).
The study of VWF was carried out by a standard enzyme-linked immunosorbent assay (ELISA) using the Human VWF ELISA Kit. Data were analyzed using Statsoft Statistica version 8 (Tulsa, OK) and MedCalc statistical software version 17.2.
Results. It was found that children with asthma had significantly increased levels of VWF in the blood serum compared to the control group. The highest levels of serum VWF were found in patients with severe asthma.
Conclusions. Elevated levels of VWF indicate the presence of endothelial dysfunction. Increased levels of VWF depending on the severity of asthma indicate more severe endothelial damage in children with severe asthma.
The research was carried out in accordance with the principles of the Declaration of Helsinki. The research protocol was approved by the Local Ethics Committee of the institution mentioned in the work. Informed consent of parents or their guardians was obtained for conducting research.
No conflict of interests was declared by the authors.
Agostini S, Lionetti V. (2017). New insights into the non-hemostatic role of von Willebrand factor in endothelial protection. The IACS 4th Cardiovascular Forum for Promoting Centers of Excellence and Young Investigators, Quebec, Canada, September 2016. Can J Physiol Pharmacol. 95: 1183-1189. https://doi.org/10.1139/cjpp-2017-0126; PMid:28715643
Eelen G, de Zeeuw P, Simons M, Carmeliet P. (2015). Endothelial cell metabolism in normal and diseased vasculature. Circ Res. 116: 1231-1244. https://doi.org/10.1161/CIRCRESAHA.116.302855; PMid:25814684 PMCid:PMC4380230
El Basset Abo El Ezz AA, Abd El Hafez MA, El Amrousy DM et al. (2017). The predictive value of Von Willebrand factor antigen plasma levels in children with acute lung injury. Pediatr Pulmonol. 52: 91-97. https://doi.org/10.1002/ppul.23518; PMid:27362747
Gheware A, Panda L, Khanna K et al. (2021, May 1). Adhatoda vasica rescues the hypoxia-dependent severe asthma symptoms and mitochondrial dysfunction. Am J Physiol Lung Cell Mol Physiol. 320 (5): L757-L769. Epub 2021 Feb 10. https://doi.org/10.1152/ajplung.00511.2020; PMid:33565386
Goncharova EA, Chan SY, Ventetuolo CE, Weissmann N, Schermuly RT, Mullin CJ, Gladwin MT. (2020). Update in pulmonary vascular diseases and right ventricular dysfunction 2019. Am J Respir Crit Care Med. 202: 22-28. https://doi.org/10.1164/rccm.202003-0576UP; PMid:32311291 PMCid:PMC7328315
Green CE, Turner AM. (2017). The role of the endothelium in asthma and chronic obstructive pulmonary disease [COPD]. Respir Res. 18: 20. https://doi.org/10.1186/s12931-017-0505-1; PMid:28100233 PMCid:PMC5241996
Guo L, Lv H, Wang J, Zhang B, Zhu Y, Zhang X et al. (2023, Apr 20). Predictive value of high sensitivity C-reactive protein in three-vessel disease patients with and without type 2 diabetes. Cardiovasc Diabetol. 22 (1): 91. https://doi.org/10.1186/s12933-023-01830-7; PMid:37081535 PMCid:PMC10120230
Hashmi MF, Tariq M, Cataletto ME. (2023). Asthma. StatPearls. Treasure Island (FL). PMID: 28613651 Bookshelf ID: NBK430901 Free Books & Documents.
Makieieva N, Butov D, Vasylchenko Y, Biriukova M, Serhiienko K, Morozov O. (2019). Endothelial Dysfunction in Children with Clinically Stable and Exacerbated Asthma. Advances in Respiratory Medicine. 87 (1): 7-13. https://doi.org/10.5603/ARM.a2019.0002; PMid:30830954
Makieieva N, Malakhova V, Vasylchenko Y, Biriukova M. (2020). Is willebrand factor indicative of chronic inflammation in children with asthma? Turk Thorac J. 21 (6): 362-366. https://doi.org/10.5152/TurkThoracJ.2019.19051; PMid:33352089 PMCid:PMC7752108
Manuyakorn W, Mairiang D, Sirachainan N, Kadegasem P, Kamchaisatian W, Benjaponpitak S, Chuansumrit A. (2016). Blood Coagulation and Asthma Exacerbation in Children. Int Arch Allergy Immunol. 170 (2): 75-83. Epub 2016 Jul 26. https://doi.org/10.1159/000446775; PMid:27454316
Manz XD, Bogaard HJ, Aman J. (2022, Nov). Regulation of VWF (Von Willebrand Factor) in Inflammatory Thrombosis. Arterioscler Thromb Vasc Biol. 42 (11): 1307-1320. Epub 2022 Sep 29. https://doi.org/10.1161/ATVBAHA.122.318179; PMid:36172866
Mojzisch A, Brehm MA. (2021, Sep 8). The Manifold Cellular Functions of von Willebrand Factor. Cells. 10 (9): 2351. https://doi.org/10.3390/cells10092351; PMid:34572000 PMCid:PMC8466076
Ochoa CD, Wu S, Stevens T. (2010, Apr). New developments in lung endothelial heterogeneity: Von Willebrand factor, P-selectin, and the Weibel-Palade body. Semin Thromb Hemost. 36 (3): 301-308. Epub 2010 May 20. https://doi.org/10.1055/s-0030-1253452; PMid:20490980 PMCid:PMC2917989
Pacholczak-Madej R, Kuszmiersz P, Iwaniec T, Zaręba L, Zarychta J, Walocha JA et al. (2021, Oct 25). Endothelial Dysfunction and Pentraxin-3 in Clinically Stable Adult Asthma Patients. J Investig Allergol Clin Immunol. 31 (5): 417-425. Epub 2020 May 6. https://doi.org/10.18176/jiaci.0563; PMid:32376513
Pi X, Xie L, Patterson C. (2018). Emerging roles of vascular endothelium in metabolic homeostasis. Circ Res. 123: 477-494. https://doi.org/10.1161/CIRCRESAHA.118.313237; PMid:30355249 PMCid:PMC6205216
Poredos P, Poredos AV, Gregoric I. (2021). Endothelial Dysfunction and Its Clinical Implications. Angiology. 72 (7): 604-615. https://doi.org/10.1177/0003319720987752; PMid:33504167
Reddel HK, Bacharier LB, Bateman ED, Brightling CE, Brusselle GG, Buhl R, Cruz AA, Duijts L, Drazen JM, FitzGerald JM, Fleming LJ, Inoue H, Ko FW, Krishnan JA, Levy ML, Lin J, Mortimer K, Pitrez PM, Sheikh A, Yorgancioglu AA, Boulet LP. (2021). The Global Initiative for Asthma Strategy 2021: executive summary and rationale for key changes. Eur Respir J. 59 (1): 2102730. https://doi.org/10.1183/13993003.02730-2021; PMid:34667060 PMCid:PMC8719459
Satta E, Alfarone C, De Maio A et al. (2022, Jan 18). Kidney and lung in pathology: mechanisms and clinical implications. Multidiscip Respir Med. 17 (2): 819. https://doi.org/10.4081/mrm.2022.819; PMid:35127080 PMCid:PMC8791019
Sneeboer MMS, Majoor CJ, de Kievit A, Meijers JCM, van der Poll T, Kamphuisen PW, Bel EH. (2016, Jun). Prothrombotic state in patients with severe and prednisolone-dependent asthma. J Allergy Clin Immunol. 137 (6): 1727-1732. Epub 2015 Dec 20. https://doi.org/10.1016/j.jaci.2015.10.038; PMid:26714414
Soh JE, Kim KM, Kwon JW, Kim HY, Seo JH, Kim HB et al. (2017, Oct 8). Recurrent wheeze and its relationship with lung function and airway inflammation in preschool children: a cross-sectional study in South Korea. BMJ Open. 7 (10): e018010. https://doi.org/10.1136/bmjopen-2017-018010; PMid:28993393 PMCid:PMC5640071
Sottile PD, Albers D, Moss MM. (2018). Neuromuscular blockade is associated with the attenuation of biomarkers of epithelial and endothelial injury in patients with moderate-to-severe acute respiratory distress syndrome. Crit Care. 22: 63. doi: https://doi.org/10.1186/s13054-018-1974-4; PMid:29523157 PMCid:PMC5845220
Taytard J, Lacin F, Nguyen TLT, Boizeau P, Alberti C, Beydon N. (2020, Jun). Children with uncontrolled asthma and significant reversibility might show hypoxaemia. Eur J Pediatr. 179 (6): 999-1005. Epub 2020 Feb 4. https://doi.org/10.1007/s00431-020-03600-z; PMid:32020332
Tinschert P, Rassouli F, Barata F et al. (2019, Jan 7). Prevalence of nocturnal cough in asthma and its potential as a marker for asthma control (MAC) in combination with sleep quality: protocol of a smartphone-based, multicentre, longitudinal observational study with two stages. BMJ Open. 9 (1): e026323. https://doi.org/10.1136/bmjopen-2018-026323; PMid:30617104 PMCid:PMC6326321
Yang X, Chang Y, Wei W. (2016). Endothelial Dysfunction and Inflammation: Immunity in Rheumatoid Arthritis. Mediators Inflamm: 6813016. Epub 2016 Mar 30. https://doi.org/10.1155/2016/6813016; PMid:27122657 PMCid:PMC4829719
Yeromenko GV, Ospanova TS, Bezditko TV. (2018). Features of profibrotic markers and endothelial diffusion in patients with bronchial asthma and comebidity with obesity and type 2 sugar diabetes. ScienceRise: Medical Science. 7 (27): 35-28. https://doi.org/10.15587/2519-4798.2018.148972
Zhang F, Yang XC, Jia XW et al. (2017). Von Willebrand factor and ADAMTS13 plasma in older patients with high CHA2DS2-VASc Score with and without atrial fibrillation. Eur Rev Med Pharmacol Sci. 21: 4907-4912.
Zhang J, Zhao L, Zhao D et al. (2019, Aug 26). Reliability and validity of the Chinese version of the Test for Respiratory and Asthma Control in Kids (TRACK) in preschool children with asthma: a prospective validation study. BMJ Open. 9 (8): e025378. https://doi.org/10.1136/bmjopen-2018-025378; PMid:31455696 PMCid:PMC6720147
Zinter MS, Delucchi KL, Kong MY et al. (2019). Early plasma matrix metalloproteinase profiles. A novel pathway in pediatric acute respiratory distress syndrome. Am J Respir Crit Care Med. 199: 181-189. https://doi.org/10.1164/rccm.201804-0678OC; PMid:30114376 PMCid:PMC6353006
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