Dynamics of gene expression of T-cells and interleukin transcription factors on the background of probiotic therapy of chronic gastroduodenitis in children


  • O.E. Abaturov Dnipro State Medical University, Ukraine, Ukraine https://orcid.org/0000-0001-6291-5386
  • N.M. Tokarieva Dnipro State Medical University, Ukraine, Ukraine
  • A.E. Lykova Dnipro State Medical University, Ukraine, Ukraine
  • O.L. Krivusha Dnipro State Medical University, Ukraine, Ukraine




chronic gastroduodenitis, children, transcription factors, interleukins, probiotic therapy, Lactobacillus rhamnosus GG


Chronic gastroduodenitis (HGD) in children is one of the most common diseases of the digestive tract in children, the treatment of which needs to be improved.

Purpose - to study the effectiveness of Lactobacillus rhamnosus GG ATCC 53103 (LGG) in the treatment of children with chronic gastroduodenitis and the effect of treatment on the expression of pro- and anti-inflammatory cytokines and T-lymphocyte transcription factors in the buccal mucosa.

Materials and methods. We examined 39 children with chronic gastroduodenitis aged 10-14 years, of which 2 comparison groups were formed. The main group (n=21) included patients for whose treatment, in addition to traditional therapy, a probiotic drug was containing 5×109 lyophilized viable bacteria LGG. The control group (n=18) was represented by patients receiving only traditional therapy. The duration of treatment with probiotic LGG was 14 days. To prove the effectiveness of LGG in therapy, the level of mRNA expression of transcription factors (Tbet, RORyt, FOXP3) of T cells and interleukins (IL-1β, IL-17α, IL-10) was determined by polymerase chain reaction with reverse transcription.

Results. The period of CGD exacerbation was characterized by an increase in the expression level of mRNA of the RORyt transcription factor of Th17-cells and mRNA of IL-1β, IL-17α in the buccal mucosa. After standard therapy in children with CHD, there was a significant increase in the activity of mRNA expression of the transcription factor Tbet of Th1-cells and a decrease in the activity of mRNA expression of the RORyt of transcription factor Th17-cells. After treatment with combination therapy using a probiotic preparation containing LGG, there was also a decrease in the activity of mRNA expression of the RORyt transcription factor of Th17-cells, but in contrast to children who received standard therapy, there was a low level of mRNA of the transcription factor Tbet of Th1-cells and mRNA IL-17α; in combination with an increased level of mRNA of the FOXP3 transcription factor of Treg-cells.

Conclusions. The appointment of a combination therapy using a probiotic preparation containing Lactobacillus rhamnosus GG in children with chronic gastroduodenitis contributes to the rapid regression of the clinical manifestations of pain, dyspeptic syndromes. The administration of LGG leads to a more rapid decrease in the level of expression of the IL-17 genes due to an increase in the activity of mRNA expression of the FOXP3 of Treg-cells.

The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local ethics committee of the participating institution. The informed consent of the patient was obtained for conducting the studies.

No conflict of interest was declared by the authors.


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