Indicators of vitamin D in case of Coronavirus infection COVID-19 in children


  • A.G. Hasanov Azerbaijan Medical University, Baku, Azerbaijan
  • I.E. Huseinova Azerbaijan Medical University, Baku, Azerbaijan



children, COVID-19, vitamin D


Purpose — to determine and compare vitamin D levels in children diagnosed with COVID-19-associated pneumonia and healthy children.

Materials and methods. The study involved 90 children. The main group consisted of 75 inpatient children with a diagnosis of COVID-19-associated pneumonia (positive PCR — polymerase chain reaction with reverse transcription). Patients in the main group were divided into 2 subgroups: subgroup I consisted of 49 patients with a pneumonia of moderate course, and subgroup II — 26 patients with a pneumonia of severe course. The control group consisted of 15 practically healthy children. The scheme of examination of sick children included anamnestic data, the use of clinical, instrumental and laboratory, immunological examination methods. The concentration of vitamin D in the blood serum of sick and healthy children was determined by the method of enzyme3linked immunosorbent assay (ELISA). The indicators were measured on a Stat Fax 4700 device using a Pishqaman reactive kit (made in Germany).

Results. The concentration of vitamin D in blood serum was 1.8 times lower in subgroup I and 2.0 times in subgroup II, compared with the control group. The average values of vitamin D in blood serum compared with the control group were <1.1 times lower in the age group <1 year, 1.1 times lower in the age group 1–3 years, and 2.3 times lower in the age group >3 years.

Conclusions. Based on our research, it can be concluded that there is a relationship between vitamin D deficiency and COVID-19. Thus, the average level of vitamin D was lower in children with COVID-19-associated pneumonia, and varied depending on the severity of the disease (more pronounced changes were observed in severe pneumonia). There are also differences in vitamin D levels in the age groups of children with COVID-19-associated pneumonia: in children from age groups under 1 year and 1–3 years old the decrease in serum vitamin D level is less pronounced than in the age group over 3 years old. The main mechanisms of vitamin D involvement in the immune modulation process in COVID-19 require further study.

The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local ethics committee of the participating institution. The informed consent of the patient was obtained for conducting the studies.

No conflict of interest was declared by the authors.


Akoglu HA et al. (2021). Evaluation of childhood COVID-19 cases: a retrospective analysis. Journal of Pediatric Infectious Diseases. 16: 91-98.

Aleksandrovich YuS, Baybarina EN, Baranov AA et al. (2020). Vedenie detey s zabolevaniem, vyizvannyim novoy koronavirusnoy infektsiey (SARS-CoV-2). Pediatricheskaya farmakologiya. 2: 103-118.

Ali N. (2020). Role of vitamin D in preventing of COVID-19 infection, progression and severity. Journal of Infection and Public Health. 13: 1373-1380.; PMid:32605780 PMCid:PMC7305922

Amaya-Mejia, AS et at. (2013). Vitamin D deficiency in patients with common variable immunodeficiency, with autoimmune diseases and bronchiectasis. Revista Alergia Mexico. 60: 110-116.

ANAS. (2020). 13 A lack of vitamin D and COVID-19 deaths have been identified. Institute of botanyplant & fungal research. ISSN 2617-8001 (Print). ISSN 2664-5297 (Online).

Aranow C. (2011). Vitamin D and the immune system. Investigative Medicine. 59: 881-886.; PMid:21527855 PMCid:PMC3166406

Aysegul A, Serkan T, Yasar K. (2021). Vitamin D levels in children with COVID-19: a report from Turkey. Cambridge University Press.

Beard JA, Bearden A, Striker R. (2011). Vitamin D and the anti-viral state. J Clin Virol. 50 (3): 194-200.; PMid:21242105 PMCid:PMC3308600

Bishop E, Ismailova A, Dimeloe SK, Hewison M, White JH. (2020). Vitamin D and immune regulation: antibacterial, antiviral, anti-inflammatory. JBMR Plus: e10405. URL:; PMid:32904944 PMCid:PMC7461279

Bradburne AF, Bynoe ML, Tyrrell DA. (1967). Effects of a «new» human respiratory virus in volunteers. Biol Med. 3: 767-769.; PMid:6043624 PMCid:PMC1843247

Carpagnano GE et al. (2020). Vitamin D deficiency as a predictor of poor prognosis in patients with acute respiratory failure due to COVID-19. Endocrinological Research.

Chan Yeung M, Xu RH. (2003). SARS: Epidemiology. Respirology. 8: 9-14.; PMid:15018127 PMCid:PMC7169193

Charan J, Goyal JP, Saxena D, Yadav P. (2012). Vitamin D for prevention of respiratory tract infections: A systematic review and meta-analysis. Pharmacol Pharmacother. 3 (4): 300-303.; PMid:23326099 PMCid:PMC3543548

Darren A et al. (2021). Vitamin D status of children with paediatric inflammatory multisystem syndrome temporally associated with severe acute respiratory syndrome coronavirus 2 (PIMS-TS). British Journal of Nutrition. 12: 1-26.; PMid:33977890 PMCid:PMC8245338

Grant WB, Lahore H, McDonnell SL, Baggerly CA, French CB, Aliano JL, Bhattoa HP. (2020). Dokazatelstva togo, chto dobavlenie vitamina D mozhet snizit risk zarazheniya i smerti grippom i COVID-19. Pitatelnyie veschestva. 12: 988.; PMid:32252338 PMCid:PMC7231123

Griffin G, Hewison M, Hopkin J, Kenny R, Quinton R, Rhodes J, Subramanian S, Thickett D. (2020). Vitamin D and COVID-19: evidence and recommendations for supplementation. R Soc Open Sci. 7: 12.; PMid:33489300 PMCid:PMC7813231

Gromova OA, Torshin IYu, Gabdulina GH. (2020). Pandemiya COVID-19: zaschitnyie roli vitamina D. Sovremennaya farmakoekonomika i farmakoepidemiologiya. 13 (2): 132-145.

Hagmann S. (2020, Mar 28). COVID-19 in children: More than meets the eye. Travel Med Infect Dis. URL: https://; PMid:32234457 PMCid:PMC7195039

Holick MF. (2006). Resurrection of vitamin D deficiency and rickets. Journal of Clinical Investigation. 116 (8): 2062-2072.; PMid:16886050 PMCid:PMC1523417

Kahn JS, McIntosh K. (2020). History and Recent Advances in Coronavirus Discovery. The Pediatric Infectious Disease. 24 (11): 223-227.; PMid:16378050

Lemire JM. (1992). Immunomodulatory role of 1.25-dihydroxyvitamin D3. Cellular Biochemistry. 49: 26-31.; PMid:1644850

Levinson M, Cevik M, Lipsitch M. (2020). Reopening Primary Schools during the Pandemic. N Engl Med. 383: 981-985.; PMid:32726550

Liu X et al. (2014). Vitamin D modulates prostaglandin E2 synthesis and degradation in human lung fibroblasts. American Journal of Respiratory Cell and Molecular Biology. 50: 40-50.; PMid:23941558

Makinen M, Mykkanen J, Koskinen M, Simell V, Veijola R, Hyoty H et al. (2016). Serum 25-hydroxyvitamin D concentrations in children progressing to autoimmunity and clinical type 1 diabetes. J Clin Endocrinol Metab. 101 (2): 723-729.; PMid:26695863 PMCid:PMC4880127

Martens PJ et al. (2020). Vitamin D's effect on immune function. Nutrients. 12: 1248.; PMid:32353972 PMCid:PMC7281985

Panfili FM et al. (2021). Possible role of vitamin D in Covid-19 infection in pediatric population. Journal of Endocrinological investigation. 44: 27-35.; PMid:32557271 PMCid:PMC7299247

Tang JW, Bahnfleth WP, Bluyssen PM et al. (2021). Dismantling myths on the airborne transmission of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Hosp Infect. 110: 89-96.; PMid:33453351 PMCid:PMC7805396

Tezer H, Bedir Demirda? T. (2020). Novel coronavirus disease (COVID-19) in children. Turkish Journal of Medical Sciences. 50: 592-603.; PMid:32304191 PMCid:PMC7195991

Vanherwegen AS, Gysemans C, Mathieu C. (2017). Regulation of Immune Function by Vitamin D and Its Use in Diseases of Immunity.Endocrinol Metab Clin North Am. 46 (4): 1061-1094.; PMid:29080635

Vanherwegen AS, Gysemans C, Mathieu C. (2017, Dec). Regulation of Immune Function by Vitamin D and Its Use in Diseases of Immunity. Endocrinol Metab Clin North Am. 46 (4): 1061-1094.; PMid:29080635

Yilmaz K, Sen V. (2020). Is vitamin D deficiency a risk factor for COVID-19 in children? Pediatr Pulmonology. 55: 3595-3601.; PMid:33017102 PMCid:PMC7675606

Zaharova IN, Klimov LYa, Kasyanova AN, Kuryaninova VA, Dolbnya SV, Ivanova AV et al. (2019). Sovremennyie predstavleniya ob immunotropnyih effektah vitamina D. Voprosyi prakticheskoy pediatrii. 14 (1): 7-17.

Zaplatnikov AL, Gorev VV. (2020). Pediatricheskie voprosyi o novoy koronavirusnoy infektsii-est li na nih segodn otvetyi? Pediatriya. Sonsilium Medicum. 1: 16-19.

Zhou Y et al. (2020). Pathogenic T cells and inflammatory monocytes inref inflammatory storm in severe COVID-19 patients. National Science Review. 7: 998-1002.; PMid:34676125 PMCid:PMC7108005





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