Using realtime polymerase chain reaction for taxonomic and quantitative analysis of multicomponent probiotics that are used in pediatrics

Authors

  • V.O. Kitam Ltd Company OD Prolisok, Ukraine
  • D.S. Yankovsky Ltd Company OD Prolisok, Ukraine
  • V.P. Shirobokov Bogomolets National Medical University, Ukraine
  • G.S. Dyment Ltd Company OD Prolisok, Ukraine
  • O.V. Litovchenko Ltd Company OD Prolisok, Ukraine
  • L.M. Shevchenko Ltd Company OD Prolisok, Ukraine
  • T.V. Shevchenko Ltd Company OD Prolisok, Ukraine

Keywords:

real-time polymerase chain reaction, DNA, multicomponent probiotic preparations, microorganism

Abstract

The methods for determining the qualitative and quantitative composition of multicomponent probiotics play an important role in their development and investigation. Both taxonomic identification and quantitative analysis of such multisymbiosis components can be significantly accelerated with application of modern methods based on the detection and identification of DNA sequences by polymerase chain reaction (PCR). One of the main requirements for this method is the purity of the isolated DNA and the absence of polymerase chain reaction inhibitors in its content. In the course of study, we developed a method for using polymerase chain reaction in real time for qualitative and quantitative determination of the species composition in multicomponent probiotic preparations containing representatives of 18 species of genera Bifidobacterium, Lactobacillus, Propionibacterium, Lactococcus, Streptococcus and Acetobacter. This method allows rapid and accurate research of samples in the presence of various inhibitors of polymerase chain reaction.

References

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Published

2020-03-28

Issue

No. 2(106) (2020): Modern pediatrics. Ukraine

Section

Reviews

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